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This lecture explains about the hotstart PCR mechanism and procedure. It also explains the hotstart PCR principle. Hotstart PCR is performed at high temperature at the starting of the PCR reaction to eliminate unwanted binding of primers. As primers are prevented to bind to the nonspecific region of the target DNA, unwanted product amplification is also prevented. So with the help of this hotstart PCR we can get more specific amplification of target DNA product.
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