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For quantification of IHC images using imageJ, please watch this video. https://www.youtube.com/watch?v=gxnWYhZ0z4k&t=620s
This video lecture describes how to quantify western blot using imagej.
1. How to upload western blot image in imagej
2. How to invert image in imagej
3. How to adjust background/ brightness and contrast of western blot image in imagej
4. How to rotate image in imagej to make the lanes in the straight line
5. How to put rectangles in the image to quantify
6. How to plot lanes in imagej
7. How to make the calculation for western blot after the quantification by calculating ratios
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