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China's Most Luxury Casino City-Macau Walk in Venetian Mall&Wynn Palace Fountain Show|Galaxy Diamond Show 2023 中国最奢华的娱乐之城|漫步在澳门威尼斯人|永利皇宫音乐喷泉秀|银河钻石灯光秀
The full name of Macau is the Macau Special Administrative Region of the People's Republic of China. It is an international free port, a world tourism and leisure center, and one of the four major casinos in the world. It has not declined, and has become one of the most economically developed and wealthy regions in the world.
澳门全称中华人民共和国澳门特别行政区，是国际自由港、世界旅游休闲中心、世界四大赌城之一，也是世界人口密度最高的地区之一，其旅游业、酒店业和博彩娱乐业使澳门长盛不衰，成为全球经济发达和富裕的地区之一。
00:00 Tour Highlights 行程概览
01:15 The Venetian Mall 威尼斯人运河购物城
10:40 St.Mark's Square 圣马可广场
18:46 Wynn Palace Fountain Show 永利皇宫音乐喷泉秀
22:26 Galaxy Lotus Light Show 银河莲花灯光秀
24:55 Galaxy Diamond Light Show 银河钻石灯光秀
The Venetian Grand Canal Shopping Center in Macau~ is the largest indoor shopping mall in Macau. It is located on the third floor of the Venetian Resort Hotel, with an area of 960,000 square feet. It gathers more than 300 merchants, dozens of restaurants and 3 indoor canals. . The entire shopping center is covered by a large lighting canopy, which can create the effect of clouds in the sky. With the characteristic streets and canals, the environment is elegant and beautiful, making tourists feel like they are in the streets of Venice, Italy, and enjoying the exotic atmosphere.
澳门威尼斯人大运河购物中心~是澳门最大型的室内购物中心，位于威尼斯人度假村酒店的三楼，面积达96万平方呎，云集超过三百多家商户、数十家餐饮店和3条室内运河。整个购物中心被一幅偌大的灯光天幕覆盖，可营造出天空的云彩效果，配合特色街道和运河，环境典雅秀丽，令旅客仿如置身意大利威尼斯的街道，尽情享受异国风情。
The Wynn Palace Musical Fountain is located on the artificial performance lake of the Wynn Palace Hotel, covering an area of 8 acres, equipped with more than 2,000 LED lights, each performance is 1-5 minutes, and the interval between each performance varies from 20 to 30 minutes. Visitors can also take Enjoy the light show on the free sightseeing cable car.
永利皇宫音乐喷泉座落于永利皇宫酒店人工表演湖上，占地8英亩，装有超过二千盏LED灯，每场表演1-5分钟，每场间隔20到30分钟不等，游客还可以乘坐免费的观光缆车欣赏灯光秀表演。
The diamond light show at Galaxy Casino in Macau, with light and shadow intertwined like a dream, is dazzling and dazzling, showing the ultimate luxury that shocks people.
澳门银河娱乐场的钻石灯光秀，光影交织如梦如幻，华彩夺目，展现了震撼人心的极致奢华。
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Dot Blot is a cheaper, easier and faster technique to detect the presence of Proteins and Nucleic Acids in a biological sample. Due to the simplicity of the technique it widely used as a ideal diagnostic tool.
This post covers the Protein Dot Blot Technique.
Dot Blot Principle
Dot Blot works based on the immunodetection principle for identifying specific protein.
Dot Blot - PrincipleDot Blot - Technique: Overview
Proteins are immobilized on to a protein binding membrane, PVDF or Nitrocellulose. After Protein Immobilization membrane is blocked to avoid non-specific binding, using blocking buffer.
Immobilized protein is then incubated with a primary antibody (for 30mins to 1 hour). The antibody is specific to the protein and is visualized either with a specific tag or with a secondary antibody.
The secondary antibody generally will carry a tag that allow the visualization of protein.
Blots can be visualized using methods such as Chemiluminescence or Fluorescence.
Common Tags used in Dot Blots are enzymes conjugated to the secondary antibody. Upon addition of substrate, enzyme acts on the substrate to develop colour. Commonly preferred enzymes are HRP(Horse Radish Peroxidase) and Alkaline Phosphatase (AP)
Advantages of Dot Blot over Western Blot
Non-Electrophoresis Technique:
Dot Blot is non-electrophoresis technique, means that one need not do protein electrophoresis. Proteins can directly immobilized on to the membrane.
Simple & Easy Method:
No messy work of PAGE run and gel to membrane transfers, proteins are directly immobilized on membrane, making this method much easier and faster.
Time:
It is a faster method, takes ~3hours, whereas western blot may even take up to 2 days. Including the protein electrophoresis and gel to membrane transfer optimization.
Dot Blot is not as informative as Western Blot, but it is inexpesive, quick and easy method for the simple protein detection.
dot blot technique by shomus biology
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